Do Ram Slots Matter Reddit

Hi Guys,
I've got an Asus P5B Deluxe motherboard. Today I tried adding some new RAM to take it from 4GB to 8GB. The board is about 6 years' old. I've never used the black RAM slots before, only the yellow ones (2x2GB in slots 1 and 3).
My system only sees 6GB, not 8GB. I've tried various combinations of the RAM, get the same result.
To check the new RAM wasn't defective, I tried it in slot 1, a known good slot. All 4 sticks of RAM worked in slot 1, so the RAM wasn't faulty.
All 4 sticks work in every slot except slot 2 (the first black RAM slot). If I put any stick in slot 2 - old or new - the PC is dead, it won't even boot to the BIOS.
So it looks like I have a defective RAM slot.
Does anyone know of any way to fix this? The board is too old to RMA. I've tried an emery board down the defective slot, and I've tried squirting WD40 down it. No improvement.
Does anyone have any other ideas on how to get that bad slot working?
Thanks!

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Ram

My motherboard has 4 ram slots. 2 of them r white and 2 of them are black. Wondering if i should plug my 2 sticks of ram into the white sockets, the black ones, or mix them. How to check RAM slots available on Windows 10. To confirm the number of memory slots available on your computer or laptop, use these steps on Windows 10. Open Start.; Search for Task Manager.

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Does it matter which slot you put an additional RAM card in?
I recently upgraded the RAM in my computer. It contained two cards in slots 1 and 3 (Or 2 and 4 depending from which side you count). This left me with two slots to add more RAM. One slot was between the two occupied ones and one on the end. I put a new 8 g card in the end or open slot as it was easier to do than putting it between the two cards that were already in there. Computer was been acting weird lately and I wonder if the new card should have gone in between the two cards that came with the computer.
The computer is a HP Pavilion 500-054 Desktop
The Memory card was recommended by Crucial